Preparation of DNA containing 7-methylguanine as unique lesions
نویسندگان
چکیده
منابع مشابه
Interaction of Murine Dnmt3a with DNA Containing O-Methylguanine
O-Methylguanine (OmeG) is one of the most toxic, mutagenic, and carcinogenic lesions caused by the interaction of DNA with several catabolism products as well as with environmental methylating agents. Carcinogenic impact of OmeG can be conditioned not only by its mutagenic properties but also by alteration in enzymatic methylation of the C5 carbon atom of cytosine residue in CpG sequences. In t...
متن کاملNovel synthesis of O-alkylguanine containing oligodeoxyribonucleotides as substrates for the human DNA repair protein, O-methylguanine DNA methyltransferase (MGMT)
The human DNA repair protein O-methylguanine DNA methyltransferase (MGMT) dealkylates mutagenic O-alkylguanine lesions within DNA in an irreversible reaction which results in inactivation of the protein. MGMT also provides resistance of tumours to alkylating agents used in cancer chemotherapy and its inactivation is therefore of particular clinical importance. We describe a post-DNA synthesis s...
متن کاملNovel synthesis of O6-alkylguanine containing oligodeoxyribonucleotides as substrates for the human DNA repair protein, O6-methylguanine DNA methyltransferase (MGMT)
The human DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) dealkylates mutagenic O6-alkylguanine lesions within DNA in an irreversible reaction which results in inactivation of the protein. MGMT also provides resistance of tumours to alkylating agents used in cancer chemotherapy and its inactivation is therefore of particular clinical importance. We describe a post-DNA synthesis...
متن کاملThe Escherichia coli MutS DNA mismatch binding protein specifically binds 0-methylguanine DNA lesions
DNA mismatch repair defects in certain cell types confer resistance to the cytotoxic effects of alkylating agents, suggesting that a normally functioning DNA mismatch repair pathway can actually mediate alkylation-induced cell death. In eukaryotic cells this phenomenon is only observed in cells lacking adequate DNA methyltransferase for the repair of 0*-methylguanine (O*MeG) DNA lesions. It has...
متن کاملThe Escherichia coli MutS DNA mismatch binding protein specifically binds O(6)-methylguanine DNA lesions.
DNA mismatch repair defects in certain cell types confer resistance to the cytotoxic effects of alkylating agents, suggesting that a normally functioning DNA mismatch repair pathway can actually mediate alkylation-induced cell death. In eukaryotic cells this phenomenon is only observed in cells lacking adequate DNA methyltransferase for the repair of O6-methylguanine (O6MeG) DNA lesions. It has...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 1999
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/42.1.83